The fault slip and nucleation of seismicity are greatly significantly affected by the asperity of the fault. The fault usually consists of three layers structures, including rock, gouge, and rock. Although the volume of gouge between rocks is very small, of the contact structure containing three layers can determine the mechanical behavior and the nucleation mode of the fault surface. A series of laboratory tests with AE monitoring were performed to study the stick slip behavior of the fault. It is found a small-scale (cm) lab test that the fault with asperity shows more evident unstable friction than the fault with a uniform structure (Bedford et al., 2022). However, whether the same pattern exists in a large-scale (dm) fault friction test has not been determined. We developed a fault friction test in a large scale with stress measurement, strain measurement, and AE monitoring. The gouge of the fault was constructed with two different materials and these materials are distributed with a spatial asperity. We used the power spectral method to fully identify microseismicity. Further, we used seismic template matching method to differentiate, classify, and organize the seismic events according to the characterization of their waveforms. We found that the loading rate was positively correlated with the occurrence frequency density of the events, and inversely correlated with the magnitude of the stick-slip seismic events. This study validated that the template matching method can be used in the laboratory experimental earthquake to identify repeating earthquakes.